Overexpression of PSR1 in Chlamydomonas reinhardtii induces luxury phosphorus uptake.

ABSTRACT: Remediation using micro-algae offers an attractive solution to environmental phosphate (PO₄ ^3-) pollution. However, for maximum efficiency, pre-conditioning of algae to induce 'luxury phosphorus (P) uptake' is needed. To replicate this process, we targeted the global regulator PSR1 (Myb transcription factor: Phosphate Starvation Response 1) for over-expression in algae. Manipulating a single gene (PSR1) drove uptake of both PO₄ ^3- and a Mg²⁺ counter-ion leading to increased PolyP granule size, raising P levels 4-fold to 8% dry cell weight, and accelerated removal of PO₄ ^3- from the medium. Examination of the gene expression profile showed that the P-starvation response was mimicked under P-replete conditions, swit

SUBMITTER: Slocombe SP 

PROVIDER: S-EPMC10413257 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature