On the basis of sex: male vs. female rat adenosine A₁/A_2A receptor affinity.

ABSTRACT:

Objective

To ensure reproducibility in biomedical research, the biological variable sex must be reported; yet a reason for using male (instead of female) rodents is seldom given. In our search for novel adenosine receptor ligands, our research group routinely determines a test compound's binding affinities at male Sprague-Dawley rat (r) adenosine A₁ and A_2A receptors via in vitro radioligand binding studies. This pilot study compared the binding affinities of four adenosine receptor ligands (frequently used as reference standards) at male and female adenosine rA₁ and rA_2A receptors.

Results

The inhibition constant (K_i) values determined using female rats correspond well to the values obtained using male rats and no marka

SUBMITTER: Janse van Rensburg HD 

PROVIDER: S-EPMC10413537 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature