Exosomes derived from mir-214-3p overexpressing mesenchymal stem cells promote myocardial repair.

ABSTRACT:

Aims

Exosomes are known as nanovesicles that are naturally secreted, playing an essential role in stem-mediated cardioprotection. This study mainly focused on investigating if exosomes derived from miR-214 overexpressing mesenchymal stem cells (MSCs) show more valid cardioprotective ability in a rat model of acute myocardial infarction (AMI) and its potential mechanisms.

Methods

Exosomes were isolated from control MSCs (Ctrl-Exo) and miR-214 overexpressing MSCs (miR-214^OE-Exo) and then they were delivered to cardiomyocytes and endothelial cells in vitro under hypoxia and serum deprivation (H/SD) condition or in vivo in an acutely infarcted Sprague-Dawley rat heart. Regulated genes and signal pathways by miR-214^OE-Exo treatment were explored using wester

SUBMITTER: Zhu W 

PROVIDER: S-EPMC10413540 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature