Chromatin opening ability of pioneer factor Pax7 depends on unique isoform and C-terminal domain.

ABSTRACT: Pioneer factors are transcription factors (TFs) that have the unique ability to recognise their target DNA sequences within closed chromatin. Whereas their interactions with cognate DNA is similar to other TFs, their ability to interact with chromatin remains poorly understood. Having previously defined the modalities of DNA interactions for the pioneer factor Pax7, we have now used natural isoforms of this pioneer as well as deletion and replacement mutants to investigate the Pax7 structural requirements for chromatin interaction and opening. We show that the GL+ natural isoform of Pax7 that has two extra amino acids within the DNA binding paired domain is unable to activate the melanotrope transcriptome and to fully activate a large subset of melanotrope-specific enhancers targeted for P

SUBMITTER: Bascunana V 

PROVIDER: S-EPMC10415112 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature