Establishing artificial gene connections through RNA displacement-assembly-controlled CRISPR/Cas9 function.

ABSTRACT: Construction of synthetic circuits that can reprogram genetic networks and signal pathways is a long-term goal for manipulation of biosystems. However, it is still highly challenging to build artificial genetic communications among endogenous RNA species due to their sequence independence and structural diversities. Here we report an RNA-based synthetic circuit that can establish regulatory linkages between expression of endogenous genes in both Escherichiacoli and mammalian cells. This design employs a displacement-assembly approach to modulate the activity of guide RNA for function control of CRISPR/Cas9. Our experiments demonstrate the great effectiveness of this RNA circuit for building artificial connections between expression of originally unrelated genes. Both exogenous and naturall

SUBMITTER: Wang WJ 

PROVIDER: S-EPMC10415155 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature