NFκB1 inhibits memory formation and supports effector function of ILC2s in memory-driven asthma.

ABSTRACT:

Background

ILC2s are capable of generating memory. The mechanism of memory induction and memory-driven effector function (trained immunity) in ILC2s is unknown.

Objective

NFκB1 is preferentially expressed at a high level in ILC2s. We examined the role of NFkB1 in memory induction and memory-driven effector function in a mouse model of asthma.

Methods

Intranasal administration of Alternaria, flexivent, ELISA, histology, real-time PCR, western blot, flow cytometry and immunofluorescence staining.

Results

NFκB1 was essential for the effector phase of memory-driven asthma. NFκB1 was critical for IL33 production, ILC2 generation, and production of type-2 cytokines, which resulted in eosinophilic inflammation and other features of asthma. NFκB1 induction of type-2 cy

SUBMITTER: Verma M 

PROVIDER: S-EPMC10415221 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature