Sarcomeric network analysis of ex vivo cultivated human atrial appendage tissue using super-resolution microscopy.

ABSTRACT: Investigating native human cardiac tissue with preserved 3D macro- and microarchitecture is fundamental for clinical and basic research. Unfortunately, the low accessibility of the human myocardium continues to limit scientific progress. To overcome this issue, utilizing atrial appendages of the human heart may become highly beneficial. Atrial appendages are often removed during open-heart surgery and can be preserved ex vivo as living tissue with varying durability depending on the culture method. In this study, we prepared living thin myocardial slices from left atrial appendages that were cultured using an air-liquid interface system for overall 10 days. Metabolic activity of the cultured slices was assessed using a conventional methyl thiazolyl tetrazolium (MTT) assay. To monitor the s

SUBMITTER: Chabanovska O 

PROVIDER: S-EPMC10415305 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature