Streamlined identification of strain engineering targets for bioprocess improvement using metabolic pathway enrichment analysis.

ABSTRACT: Metabolomics is a powerful tool for the identification of genetic targets for bioprocess optimisation. However, in most cases, only the biosynthetic pathway directed to product formation is analysed, limiting the identification of these targets. Some studies have used untargeted metabolomics, allowing a more unbiased approach, but data interpretation using multivariate analysis is usually not straightforward and requires time and effort. Here we show, for the first time, the application of metabolic pathway enrichment analysis using untargeted and targeted metabolomics data to identify genetic targets for bioprocess improvement in a more streamlined way. The analysis of an Escherichia coli succinate production bioprocess with this methodology revealed three significantly modulated pathways

SUBMITTER: Cortada-Garcia J 

PROVIDER: S-EPMC10415327 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature