Oral squamous carcinoma cell lysates provoke exacerbated inflammatory response in gingival fibroblasts.

ABSTRACT:

Objectives

To study whether damaged epithelial cells and gingival fibroblast could affect the expression of inflammatory cytokines in healthy cells.

Materials and methods

Cell suspensions were submitted to different treatments to obtain the lysates: no treatment (supernatant control), sonication, and freeze/thawing. All treatments were centrifuged, and the supernatants of the lysates were used for experimentation. Cell viability assays, RT-qPCR of IL1, IL6 and IL8, IL6 immunoassay, and immunofluorescence of NF-kB p65 were applied to verify the inflammatory crosstalk of damaged cells over healthy plated cells. Furthermore, titanium discs and collagen membranes were treated with lysates and checked for IL8 expression by RT-qPCR.

Results

Lysates obtained upon sonication

SUBMITTER: Sordi MB 

PROVIDER: S-EPMC10415472 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature