Subcellular localization of Na⁺/K⁺-ATPase isoforms resolved by in situ hybridization chain reaction in the gill of chum salmon at freshwater and seawater.

ABSTRACT: The Na⁺/K⁺-ATPase (NKA) α1-isoforms were examined by in situ hybridization chain reaction (ISHCR) using short hairpin DNAs, and we showed triple staining of NKA α1a, α1b, and α1c transcripts in the gill of chum salmon acclimated to freshwater (FW) and seawater (SW). The NKA α1-isoforms have closely resembled nucleotide sequences, which could not be differentiated by conventional in situ hybridization. The ISHCR uses a split probe strategy to allow specific hybridization using regular oligo DNA, resulting in high specificity at low cost. The results showed that NKA α1c was expressed ubiquitously in gill tissue and no salinity effects were observed. FW lamellar ionocytes (type-I ionocytes) expressed cytoplasmic NKA α1a and nuclear NKA α1b transcripts. However, both tran

SUBMITTER: Wong MKS 

PROVIDER: S-EPMC10415477 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature