Identification of an immunodominant IgE epitope of Der f 40, a novel allergen of Dermatophagoides farinae

ABSTRACT:

Background

House dust mites (HDMs), including Dermatophagoides pteronyssinus (Der p) and Dermatophagoides farinae (Der f) species, represent a major source of inhalant allergens that induce IgE-mediated anaphylactic reactions. HDM allergen identification is important to the diagnosis and treatment of allergic diseases. Here, we report the identification of a novel HDM allergen, which we suggest naming Der f 40, and its immunodominant IgE epitopes.

Methods

The recombinant protein Der f 40 was expressed using a pET prokaryotic expression system and purified with Ni-NTA resins. IgE binding activity was evaluated by IgE-western blot, dot-blot, and ELISA. Mast cell activation testing was performed to assess the cellular effects of IgE binding in mouse bone marrow derived mast

SUBMITTER: Cai Z 

PROVIDER: S-EPMC10415791 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature