5-HT_1A and 5-HT_2B receptor interaction and co-clustering regulate serotonergic neuron excitability.

ABSTRACT: Many psychiatric diseases have been associated with serotonin (5-HT) neuron dysfunction. The firing of 5-HT neurons is known to be under 5-HT_1A receptor-mediated autoinhibition, but functional consequences of coexpressed receptors are unknown. Using co-immunoprecipitation, BRET, confocal, and super-resolution microscopy in hippocampal and 5-HT neurons, we present evidence that 5-HT_1A and 5-HT_2B receptors can form heterodimers and co-cluster at the plasma membrane of dendrites. Selective agonist stimulation of coexpressed 5-HT_1A and 5-HT_2B receptors prevents 5-HT_1A receptor internalization and increases 5-HT_2B receptor membrane clustering. Current clamp recordings of 5-HT neurons revealed that 5-HT_1A rec

SUBMITTER: Benhadda A 

PROVIDER: S-EPMC10415917 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature