A Peptidisc-Based Survey of the Plasma Membrane Proteome of a Mammalian Cell.

ABSTRACT: Membrane proteins play critical roles at the cell surface and their misfunction is a hallmark of many human diseases. A precise evaluation of the plasma membrane proteome is therefore essential for cell biology and for discovering novel biomarkers and therapeutic targets. However, the low abundance of this proteome relative to soluble proteins makes it difficult to characterize, even with the most advanced proteomics technologies. Here, we apply the peptidisc membrane mimetic to purify the cell membrane proteome. Using the HeLa cell line as a reference, we capture 500 different integral membrane proteins, with half annotated to the plasma membrane. Notably, the peptidisc library is enriched with several ABC, SLC, GPCR, CD, and cell adhesion molecules that generally exist at low to very low

SUBMITTER: Zhao Z 

PROVIDER: S-EPMC10416069 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature