Crystal Structure of de Novo Designed Coiled-Coil Protein Origami Triangle.

ABSTRACT: Coiled-coil protein origami (CCPO) uses modular coiled-coil building blocks and topological principles to design polyhedral structures distinct from those of natural globular proteins. While the CCPO strategy has proven successful in designing diverse protein topologies, no high-resolution structural information has been available about these novel protein folds. Here we report the crystal structure of a single-chain CCPO in the shape of a triangle. While neither cyclization nor the addition of nanobodies enabled crystallization, it was ultimately facilitated by the inclusion of a GCN₂ homodimer. Triangle edges are formed by the orthogonal parallel coiled-coil dimers P1:P2, P3:P4, and GCN₂ connected by short linkers. A triangle has a large central cavity and is additi

SUBMITTER: Satler T 

PROVIDER: S-EPMC10416210 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature