CCR2 macrophage response determines the functional outcome following cardiomyocyte transplantation.

ABSTRACT:

Background

The immune response is a crucial factor for mediating the benefit of cardiac cell therapies. Our previous research showed that cardiomyocyte transplantation alters the cardiac immune response and, when combined with short-term pharmacological CCR2 inhibition, resulted in diminished functional benefit. However, the specific role of innate immune cells, especially CCR2 macrophages on the outcome of cardiomyocyte transplantation, is unclear.

Methods

We compared the cellular, molecular, and functional outcome following cardiomyocyte transplantation in wildtype and T cell- and B cell-deficient Rag2^del mice. The cardiac inflammatory response was assessed using flow cytometry. Gene expression profile was assessed using single-cell and bulk RNA sequencing. Card

SUBMITTER: Vasudevan P 

PROVIDER: S-EPMC10416392 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature