DNA Volume, Topology, and Flexibility Dictate Nanopore Current Signals.

ABSTRACT: Nanopores have developed into powerful single-molecule sensors capable of identifying and characterizing small polymers, such as DNA, by electrophoretically driving them through a nanoscale pore and monitoring temporary blockades in the ionic pore current. However, the relationship between nanopore signals and the physical properties of DNA remains only partly understood. Herein, we introduce a programmable DNA carrier platform to capture carefully designed DNA nanostructures. Controlled translocation experiments through our glass nanopores allowed us to disentangle this relationship. We vary DNA topology by changing the length, strand duplications, sequence, unpaired nucleotides, and rigidity of the analyte DNA and find that the ionic current drop is mainly determined by the volume and fl

SUBMITTER: Li Y 

PROVIDER: S-EPMC10416563 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature