Inducible Rbpms-CreER^T2 Mouse Line for Studying Gene Function in Retinal Ganglion Cell Physiology and Disease.

ABSTRACT: Retinal ganglion cells (RGCs) are the sole output neurons conveying visual stimuli from the retina to the brain, and dysfunction or loss of RGCs is the primary determinant of visual loss in traumatic and degenerative ocular conditions. Currently, there is a lack of RGC-specific Cre mouse lines that serve as invaluable tools for manipulating genes in RGCs and studying the genetic basis of RGC diseases. The RNA-binding protein with multiple splicing (RBPMS) is identified as the specific marker of all RGCs. Here, we report the generation and characterization of a knock-in mouse line in which a P2A-CreER^T2 coding sequence is fused in-frame to the C-terminus of endogenous RBPMS, allowing for the co-expression of RBPMS and CreER^T2. The inducible Rbpms-CreER^T2

SUBMITTER: Guo L 

PROVIDER: S-EPMC10416940 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature