Salivary micro RNAs as biomarkers for oropharyngeal cancer.

ABSTRACT:

Background

Despite the rising incidence, particularly of the human papillomavirus (HPV)-associated fraction of oropharyngeal cancer (OPC), there are no early detection methods for OPC. Considering the close association between saliva and head and neck cancers, this study was designed to investigate salivary micro RNA (miRNAs) associated with OPC, especially focusing on HPV-positive OPC.

Methods

Saliva was collected from OPC patients at diagnosis and patients were clinically followed up ≤5 years. Salivary small RNA isolated from HPV-positive OPC patients (N = 6), and HPV-positive (N = 4) and negative controls (N = 6) were analysed by next-generation sequencing to identify dysregulated miRNAs. Discovered miRNAs were validated by quantitative PCR using two different assays in a

SUBMITTER: Ekanayake Weeramange C 

PROVIDER: S-EPMC10417169 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature