Cellular and Genomic Features of Muscle Differentiation from Isogenic Fibroblasts and Myoblasts.

ABSTRACT: The ability to recapitulate muscle differentiation in vitro enables the exploration of mechanisms underlying myogenesis and muscle diseases. However, obtaining myoblasts from patients with neuromuscular diseases or from healthy subjects poses ethical and procedural challenges that limit such investigations. An alternative consists in converting skin fibroblasts into myogenic cells by forcing the expression of the myogenic regulator MYOD. Here, we directly compared cellular phenotype, transcriptome, and nuclear lamina-associated domains (LADs) in myo-converted human fibroblasts and myotubes differentiated from myoblasts. We used isogenic cells from a 16-year-old donor, ruling out, for the first time to our knowledge, genetic factors as a source of variations between the two myogenic models.

SUBMITTER: Benarroch L 

PROVIDER: S-EPMC10417614 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature