MicroRNAs Regulate Ca²⁺ Homeostasis in Murine Embryonic Stem Cells.

ABSTRACT: MicroRNAs (miRNAs) are important regulators of embryonic stem cell (ESC) biology, and their study has identified key regulatory mechanisms. To find novel pathways regulated by miRNAs in ESCs, we undertook a bioinformatics analysis of gene pathways differently expressed in the absence of miRNAs due to the deletion of Dicer, which encodes an RNase that is essential for the synthesis of miRNAs. One pathway that stood out was Ca²⁺ signaling. Interestingly, we found that Dicer^-/- ESCs had no difference in basal cytoplasmic Ca²⁺ levels but were hyperresponsive when Ca²⁺ import into the endoplasmic reticulum (ER) was blocked by thapsigargin. Remarkably, the increased Ca²⁺ response to thapsigargin in ESCs resulted in almost no in

SUBMITTER: Reid KM 

PROVIDER: S-EPMC10417630 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature