Leucine suppresses glucagon secretion from pancreatic islets by directly modulating α-cell cAMP.

ABSTRACT:

Objective

Pancreatic islets are nutrient sensors that regulate organismal blood glucose homeostasis. Glucagon release from the pancreatic α-cell is important under fasted, fed, and hypoglycemic conditions, yet metabolic regulation of α-cells remains poorly understood. Here, we identified a previously unexplored role for physiological levels of leucine, which is classically regarded as a β-cell fuel, in the intrinsic regulation of α-cell glucagon release.

Methods

GcgCre^ERT:CAMPER and GcgCre^ERT:GCaMP6s mice were generated to perform dynamic, high-throughput functional measurements of α-cell cAMP and Ca²⁺ within the intact islet. Islet perifusion assays were used for simultaneous, time-resolved measurements of glucagon and insulin release from m

SUBMITTER: Knuth ER 

PROVIDER: S-EPMC10418066 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature