Divergent patterns of healthy aging across human brain regions at single-cell resolution reveal links to neurodegenerative disease.

ABSTRACT: Age is a major common risk factor underlying neurodegenerative diseases, including Alzheimer's disease, Parkinson's disease, and amyotrophic lateral sclerosis. Previous studies reported that chronological age correlates with differential gene expression across different brain regions. However, prior datasets have not disambiguated whether expression associations with age are due to changes in cell numbers and/or gene expression per cell. In this study, we leveraged single nucleus RNA-sequencing (snRNAseq) to examine changes in cell proportions and transcriptomes in four different brain regions, each from 12 donors aged 20-30 years (young) or 60-85 years (old). We sampled 155,192 nuclei from two cortical regions (entorhinal cortex and middle temporal gyrus) and two subcortical regions (puta

SUBMITTER: Duffy MF 

PROVIDER: S-EPMC10418086 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature