Modeling Allosteric Mechanisms of Eukaryotic Type II Topoisomerases.

ABSTRACT: Type II topoisomerases (TopoIIs) are ubiquitous enzymes that are involved in crucial nuclear processes such as genome organization, chromosome segregation, and other DNA metabolic processes. These enzymes function as large, homodimeric complexes that undergo a complex cycle of binding and hydrolysis of two ATP molecules in their ATPase domains, which regulates the capture and passage of one DNA double-helix through a second, cleaved DNA molecule. This process requires the transmission of information about the state of the bound nucleotide over vast ranges in the TopoII complex. How this information is transmitted at the molecular level to regulate TopoII functions and how protein substitutions disrupt these mechanisms remains largely unknown. Here, we employed extensive microsecond scale m

SUBMITTER: Evoli S 

PROVIDER: S-EPMC10418245 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature