Predicting the Effect of Single Mutations on Protein Stability and Binding with Respect to Types of Mutations.

ABSTRACT: The development of methods and algorithms to predict the effect of mutations on protein stability, protein-protein interaction, and protein-DNA/RNA binding is necessitated by the needs of protein engineering and for understanding the molecular mechanism of disease-causing variants. The vast majority of the leading methods require a database of experimentally measured folding and binding free energy changes for training. These databases are collections of experimental data taken from scientific investigations typically aimed at probing the role of particular residues on the above-mentioned thermodynamic characteristics, i.e., the mutations are not introduced at random and do not necessarily represent mutations originating from single nucleotide variants (SNV). Thus, the reported performance

SUBMITTER: Pandey P 

PROVIDER: S-EPMC10418460 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature