A Bright, Photostable Dye that Enables Multicolor, Time Lapse, and Super-Resolution Imaging of Acidic Organelles.

ABSTRACT: Lysosomes have long been known for their acidic lumen and efficient degradation of cellular byproducts. In recent years it has become clear that their function is far more sophisticated, involving multiple cell signaling pathways and interactions with other organelles. Unfortunately, their acidic interior, fast dynamics, and small size makes lysosomes difficult to image with fluorescence microscopy. Here we report a far-red small molecule, HMSiR ₆₈₀ -Me, that fluoresces only under acidic conditions, causing selective labeling of acidic organelles in live cells. HMSiR ₆₈₀ -Me can be used alongside other far-red dyes in multicolor imaging experiments and is superior to existing lysosome probes in terms of photostability and maintaining cell health and lyso-some motility

SUBMITTER: Lesiak L 

PROVIDER: S-EPMC10418513 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature