Proteomic Analysis of Dysfunctional Liver Sinusoidal Endothelial Cells Reveals Substantial Differences in Most Common Experimental Models of Chronic Liver Diseases.

ABSTRACT: Molecular markers of dedifferentiation of dysfunctional liver sinusoidal endothelial cells (LSEC) have not been fully elucidated. We aimed at deciphering the molecular profile of dysfunctional LSEC in different pathological scenarios. Flow cytometry was used to sort CD11b^-/CD32b⁺ and CD11b^-/CD32b^- LSEC from three rat models of liver disease (bile duct ligation-BDL; inhaled carbon tetrachloride-CCl4; and high fat glucose/fructose diet-HFGFD). A full proteomic profile was performed applying nano-scale liquid chromatography tandem mass spectrometry (nLC-MS) and analyzed with PEAKS software. The percentage of CD32b^- LSEC varied across groups, suggesting different capillarization processes. Both CD32⁺ and CD32b^- LSEC f

SUBMITTER: Gil M 

PROVIDER: S-EPMC10418749 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature