Dataset Information

Opportunities and Challenges of In Vitro Tissue Culture Systems in the Era of Crop Genome Editing.

ABSTRACT: Currently, the development of genome editing (GE) tools has provided a wide platform for targeted modification of plant genomes. However, the lack of versatile DNA delivery systems for a large variety of crop species has been the main bottleneck for improving crops with beneficial traits. Currently, the generation of plants with heritable mutations induced by GE tools mostly goes through tissue culture. Unfortunately, current tissue culture systems restrict successful results to only a limited number of plant species and genotypes. In order to release the full potential of the GE tools, procedures need to be species and genotype independent. This review provides an in-depth summary and insights into the various in vitro tissue culture systems used for GE in the economically important crops barley, wheat, rice, sorghum, soybean, maize, potatoes, cassava, and millet and uncovers new opportunities and challenges of already-established tissue culture platforms for GE in the crops.

SUBMITTER: Bekalu ZE

PROVIDER: S-EPMC10419073 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature

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Opportunities and Challenges of In Vitro Tissue Culture Systems in the Era of Crop Genome Editing.

Bekalu Zelalem Eshetu ZE Panting Michael M Bæksted Holme Inger I Brinch-Pedersen Henrik H

International journal of molecular sciences 20230725 15

Currently, the development of genome editing (GE) tools has provided a wide platform for targeted modification of plant genomes. However, the lack of versatile DNA delivery systems for a large variety of crop species has been the main bottleneck for improving crops with beneficial traits. Currently, the generation of plants with heritable mutations induced by GE tools mostly goes through tissue culture. Unfortunately, current tissue culture systems restrict successful results to only a limited n ...[more]

PMID: 37569295

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Dataset Information

Opportunities and Challenges of In Vitro Tissue Culture Systems in the Era of Crop Genome Editing.

Publications

Opportunities and Challenges of In Vitro Tissue Culture Systems in the Era of Crop Genome Editing.

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