Recapitulating the Drifting and Fusion of Two-Generation Spheroids on Concave Agarose Microwells.

ABSTRACT: Cells with various structures and proteins naturally come together to cooperate in vivo. This study used cell spheroids cultured in agarose micro-wells as a 3D model to study the movement of cells or spheroids toward other spheroids. The formation dynamics of tumor spheroids and the interactions of two batches of cells in the agarose micro-wells were studied. The results showed that a concave bottom micro-well (diameter: 2 mm, depth: 2 mm) prepared from 3% agarose could be used to study the interaction of two batches of cells. The initial tumor cell numbers from 5 × 10³ cells/well to 6 × 10⁴ cells/well all could form 3D spheroids after 3 days of incubation. Adding the second batch of DU 145 cells to the existing DU 145 spheroid resulted in the formation of satellite c

SUBMITTER: Pan R 

PROVIDER: S-EPMC10419262 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature