Tag and Snag: A New Platform for Bioactive Natural Product Screening from Mixtures.

ABSTRACT: Natural products provide an unparalleled diversity of small molecules to fuel drug screening efforts, but deconvoluting the pharmacological activity of natural product mixtures to identify key bioactive compounds remains a vexing and labor-intensive process. Therefore, we have developed a new platform to probe the non-specific pharmacological potential of compounds present in common dietary supplements via shotgun derivatization with isotopically labeled propanoic acid, a live cell affinity assay, which was used to selectively recognize the population of compounds which bind tightly to HeLa cells in culture, and a computational LC-MS data analysis of isotopically labeled compounds from cell lysate. The data analysis showed that hundreds of compounds were successfully derivatized in each ex

SUBMITTER: Seidel J 

PROVIDER: S-EPMC10421080 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature