Preliminary Study on Rapid and Simultaneous Detection of Viable Escherichia coli O157:H7, Staphylococcus aureus, and Salmonella by PMA-mPCR in Food.

ABSTRACT: Escherichia coli O157:H7, Staphylococcus aureus, and Salmonella are major foodborne pathogens that are widespread in nature and responsible for several outbreaks of food safety accidents. Thus, a rapid and practical technique (PMA-mPCR) was developed for the simultaneous detection of viable E. coli O157:H7, S. aureus, and Salmonella in pure culture and in a food matrix. To eliminate false positive results, propidium monoazide (PMA) was applied to selectively suppress the DNA amplification of dead cells. The results showed the optimum concentration of PMA is 5.0 µg/mL. The detection limit of this assay by mPCR was 10³ CFU/mL in the culture broth, and by PMA-mPCR was 10⁴ CFU/mL both in pure culture and a food matrix (milk and gr

SUBMITTER: Liu Y 

PROVIDER: S-EPMC10421101 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature