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The Small RNA Component of Arabidopsis thaliana Phloem Sap and Its Response to Iron Deficiency.

ABSTRACT: In order to discover sRNA that might function during iron deficiency stress, RNA was prepared from phloem exudates of Arabidopsis thaliana, and used for RNA-seq. Bioanalyzer results indicate that abundant RNA from phloem is small in size-less than 200 nt. Moreover, typical rRNA bands were not observed. Sequencing of eight independent phloem RNA samples indicated that tRNA-derived fragments, specifically 5' tRFs and 5' tRNA halves, are highly abundant in phloem sap, comprising about 46% of all reads. In addition, a set of miRNAs that are present in phloem sap was defined, and several miRNAs and sRNAs were identified that are differentially expressed during iron deficiency.

SUBMITTER: Bakirbas A

PROVIDER: S-EPMC10421156 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature

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The Small RNA Component of Arabidopsis thaliana Phloem Sap and Its Response to Iron Deficiency.

Bakirbas Ahmet A Castro-Rodriguez Rosario R Walker Elsbeth L EL

Plants (Basel, Switzerland) 20230727 15

In order to discover sRNA that might function during iron deficiency stress, RNA was prepared from phloem exudates of Arabidopsis thaliana, and used for RNA-seq. Bioanalyzer results indicate that abundant RNA from phloem is small in size-less than 200 nt. Moreover, typical rRNA bands were not observed. Sequencing of eight independent phloem RNA samples indicated that tRNA-derived fragments, specifically 5' tRFs and 5' tRNA halves, are highly abundant in phloem sap, comprising about 46% of ...[more]

PMID: 37570935

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