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Solid-phase capture and profiling of open chromatin by spatial ATAC.

ABSTRACT: Current methods for epigenomic profiling are limited in their ability to obtain genome-wide information with spatial resolution. We introduce spatial ATAC, a method that integrates transposase-accessible chromatin profiling in tissue sections with barcoded solid-phase capture to perform spatially resolved epigenomics. We show that spatial ATAC enables the discovery of the regulatory programs underlying spatial gene expression during mouse organogenesis, lineage differentiation and in human pathology.

SUBMITTER: Llorens-Bobadilla E

PROVIDER: S-EPMC10421738 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature

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Solid-phase capture and profiling of open chromatin by spatial ATAC.

Llorens-Bobadilla Enric E Zamboni Margherita M Marklund Maja M Bhalla Nayanika N Chen Xinsong X Hartman Johan J Frisén Jonas J Ståhl Patrik L PL

Nature biotechnology 20230105 8

Current methods for epigenomic profiling are limited in their ability to obtain genome-wide information with spatial resolution. We introduce spatial ATAC, a method that integrates transposase-accessible chromatin profiling in tissue sections with barcoded solid-phase capture to perform spatially resolved epigenomics. We show that spatial ATAC enables the discovery of the regulatory programs underlying spatial gene expression during mouse organogenesis, lineage differentiation and in human patho ...[more]

PMID: 36604544

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