Raman Spectroscopic Analysis of Highly-Concentrated Antibodies under the Acid-Treated Conditions.

ABSTRACT:

Purpose

Antibody drugs are usually formulated as highly-concentrated solutions, which would easily generate aggregates, resulting in loss of efficacy. Although low pH increases the colloidal dispersion of antibodies, acid denaturation can be an issue. Therefore, knowing the physical properties at low pH under high concentration conditions is important.

Methods

Raman spectroscopy was used to investigate pH-induced conformational changes of antibodies at 50 mg/ml. Experiments in pH 3 to 7 were performed for human serum IgG and recombinant rituximab.

Results

We detected the evident changes at pH 3 in Tyr and Trp bands, which are the sensitive markers of intermolecular interactions. Thermal transition analysis over the pH range demonstrated that the thermal transition tem

SUBMITTER: Sato Y 

PROVIDER: S-EPMC10421790 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature