HDAC5 modulates SATB1 transcriptional activity to promote lung adenocarcinoma.

ABSTRACT:

Background

Dysregulation of histone deacetylases has been linked to diverse cancers. HDAC5 is a histone deacetylase belonging to Class IIa family of histone deacetylases. Limited substrate repertoire restricts the understanding of molecular mechanisms underlying its role in tumorigenesis.

Methods

We employed a biochemical screen to identify SATB1 as HDAC5-interacting protein. Coimmunoprecipitation and deacetylation assay were performed to validate SATB1 as a HDAC5 substrate. Proliferation, migration assay and xenograft studies were performed to determine the effect of HDAC5-SATB1 interaction on tumorigenesis.

Results

Here we report that HDAC5 binds to and deacetylates SATB1 at the conserved lysine 411 residue. Furthermore, dynamic regulation of acetylation at this sit

SUBMITTER: Sharma S 

PROVIDER: S-EPMC10421875 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature