Differences in DNA methylation of HAMP in blood cells predicts the development of type 2 diabetes.

ABSTRACT:

Objectives

Better disease management can be achieved with earlier detection through robust, sensitive, and easily accessible biomarkers. The aim of the current study was to identify novel epigenetic biomarkers determining the risk of type 2 diabetes (T2D).

Methods

Livers of 10-week-old female New Zealand Obese (NZO) mice, slightly differing in their degree of hyperglycemia and liver fat content and thereby in their diabetes susceptibility were used for expression and methylation profiling. We screened for differences in hepatic expression and DNA methylation in diabetes-prone and -resistant mice, and verified a candidate (HAMP) in human livers and blood cells. Hamp expression was manipulated in primary hepatocytes and insulin-stimulated pAKT was detected. Luciferase reporter

SUBMITTER: Ouni M 

PROVIDER: S-EPMC10422014 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature