Strategies for Mitigating Commercial Sensor Chip Variability with Experimental Design Controls.

ABSTRACT: Surface plasmon resonance (SPR) is a popular real-time technique for the measurement of binding affinity and kinetics, and bench-top instruments combine affordability and ease of use with other benefits of the technique. Biomolecular ligands labeled with the 6xHis tag can be immobilized onto sensing surfaces presenting the Ni²⁺-nitrilotriacetic acid (NTA) functional group. While Ni-NTA immobilization offers many advantages, including the ability to regenerate and reuse the sensors, its use can lead to signal variability between experimental replicates. We report here a study of factors contributing to this variability using the Nicoya OpenSPR as a model system and suggest ways to control for those factors, increasing the reproducibility and rigor of the data. Our model ligand/an

SUBMITTER: Hanson EK 

PROVIDER: S-EPMC10422579 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature