Comparison of four DNA extraction methods for 16s rRNA microbiota profiling of human faecal samples.

ABSTRACT:

Objective

Growth in large population-based studies assessing contributions of the gut microbiota to health and disease requires high-throughput sample processing and analysis methods. This study assessed the impact that modifications to a commercially available magnetic bead based, semi-automated DNA extraction kit had on determination of microbial composition, relative to an established in-house method involving a combination of mechanical and chemical lysis. DNA was extracted from faecal samples from healthy adults (n = 12; 34-69 years), microbial composition was determined by V3-V4 16s rRNA sequencing and compared between extraction methods.

Results

Diversity metrics did not differ between extraction methods. Differences in the relative abundance of key phyla, including a

SUBMITTER: Sinclair J 

PROVIDER: S-EPMC10422837 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature