Non-invasive prenatal testing for fetal Ss, Kidd, and CTL2 blood group prediction by multiplex digital droplet PCR.

ABSTRACT:

Background

Some blood groups, such as S and s blood groups in the MNS blood group system, and Kidd and CTL2 blood group systems, can cause severe fetal and newborn alloimmune disorders. Non-invasive prenatal testing (NIPT) to predict fetal blood groups and knowledge of local blood group gene frequency are both important for pregnancy management decisions. Droplet digital PCR (ddPCR) has high specificity and sensitivity in detecting fetal single nucleotide variation.

Objectives

The objective is to predict fetal Ss, Kidd, and CTL2 blood groups using multiplex ddPCR. The gene frequencies of three blood groups were detected by ddPCR in northwest China.

Design

This is a prospective study.

Methods

Cell-free fetal DNA isolated from 26 healthy single pregnant women at

SUBMITTER: Wang Y 

PROVIDER: S-EPMC10422893 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature