Molecular mechanism underlying regulation of Arabidopsis CLCa transporter by nucleotides and phospholipids.

ABSTRACT: Chloride channels (CLCs) transport anion across membrane to regulate ion homeostasis and acidification of intracellular organelles, and are divided into anion channels and anion/proton antiporters. Arabidopsis thaliana CLCa (AtCLCa) transporter localizes to the tonoplast which imports NO₃^- and to a less extent Cl^- from cytoplasm. The activity of AtCLCa and many other CLCs is regulated by nucleotides and phospholipids, however, the molecular mechanism remains unclear. Here we determine the cryo-EM structures of AtCLCa bound with NO₃^- and Cl^-, respectively. Both structures are captured in ATP and PI(4,5)P₂ bound conformation. Structural and electrophysiological analyses reveal a previously unidentified N-terminal β-

SUBMITTER: Yang Z 

PROVIDER: S-EPMC10423218 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature