The PcMYB44-mediated miR397-PcLACs module regulates defence-induced lignification in pear resistance to fungal disease.

ABSTRACT: Diseases caused by Alternaria alternata and Botryosphaeria dothidea diminish pear yield and quality, and restrict the pear agricultural industry. Lignification is a conserved mechanism for plant resistance against pathogen invasion. The regulatory mechanisms underlying defence-induced lignification in pear in response to fungal pathogen infection remain unknown. In this study, analysis of lignification level and lignin content in pear revealed that A. alternata and B. dothidea induced lignification, and transcriptomics showed that lignin biosynthesis was affected. To explore whether laccases (LACs) mediated by miR397 regulate lignification in pear, we investigated the role of PcmiR397 in repressing the expression of PcLACs using 5'-RNA ligase-mediated-RACE and co-transformation in tobacco.

SUBMITTER: Yang Y 

PROVIDER: S-EPMC10423334 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature