A high throughput screening process and quick isolation of novel lignin-degrading microbes from large number of natural biomasses.

ABSTRACT: High throughput screening approaches can significantly speed up the identification of novel enzymes from natural microbial consortiums. A two-step high throughput screening process was proposed and explored to screen lignin-degrading microorganisms. By employing this modified culture enrichment method and screening based on enzyme activity, a total of 82 bacterial and 46 fungal strains were isolated from fifty decayed wood samples (100 liquid cultures) collected from the banks of the Ottawa River in Canada. Among them, ten bacterial and five fungal strains were selected and identified based on their high laccase activities by 16S rDNA and ITS gene sequencing, respectively. The study identified bacterial strains from various genera including Serratia, Enterobacter, Raoultella, and

SUBMITTER: Ali NS 

PROVIDER: S-EPMC10423689 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature