Post-Translational Modification β-Hydroxybutyrylation Regulates Ustilaginoidea virens Virulence.

ABSTRACT: Lysine β-hydroxybutyrylation (K_bhb) is an evolutionarily conserved and widespread post-translational modification that is associated with active gene transcription and cellular proliferation. However, its role in phytopathogenic fungi remains unknown. Here, we characterized K_bhb in the rice false smut fungus Ustilaginoidea virens. We identified 2204 K_bhb sites in 852 proteins, which are involved in diverse biological processes. The mitogen-activated protein kinase UvSlt2 is a K_bhb protein, and a strain harboring a point mutation at K72, the K_bhb site of this protein, had decreased UvSlt2 activity and reduced fungal virulence. Molecular dynamic simulations revealed that K72_bhb increases the hydrophobic solvent-accessible surf

SUBMITTER: Chen X 

PROVIDER: S-EPMC10423879 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature