Deubiquitinase USP9X stabilizes RNA m⁶A demethylase ALKBH5 and promotes acute myeloid leukemia cell survival.

ABSTRACT: Post-translational modifications including protein ubiquitination regulate a plethora of cellular processes in distinct manners. RNA N⁶-methyladenosine is the most abundant post-transcriptional modification on mammalian mRNAs and plays important roles in various physiological and pathological conditions including hematologic malignancies. We previously determined that the RNA N⁶-methyladenosine eraser ALKBH5 is necessary for the maintenance of acute myeloid leukemia (AML) stem cell function, but the post-translational modifications involved in ALKBH5 regulation remain elusive. Here, we show that deubiquitinase ubiquitin-specific peptidase 9X (USP9X) stabilizes ALKBH5 and promotes AML cell survival. Through the use of mass spectrometry as an unbiased approach, we ident

SUBMITTER: Wang P 

PROVIDER: S-EPMC10424212 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature