Structural and spectroscopic characterization of RufO indicates a new biological role in rufomycin biosynthesis.

ABSTRACT: Rufomycins constitute a class of cyclic heptapeptides isolated from actinomycetes. They are secondary metabolites that show promising treatment against Mycobacterium tuberculosis infections by inhibiting a novel drug target. Several nonproteinogenic amino acids are integrated into rufomycins, including a conserved 3-nitro-tyrosine. RufO, a cytochrome P450 (CYP)-like enzyme, was proposed to catalyze the formation of 3-nitro-tyrosine in the presence of O₂ and NO. To define its biological function, the interaction between RufO and the proposed substrate tyrosine is investigated using various spectroscopic methods that are sensitive to the structural change of a heme center. However, a low- to high-spin state transition and a dramatic increase in the redox potential that are commonl

SUBMITTER: Jordan S 

PROVIDER: S-EPMC10424215 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature