Novel human lymph node-derived matrix supports the adhesion of metastatic oral carcinoma cells.

ABSTRACT:

Background

3D culture is increasingly used in cancer research, as it allows the growth of cells in an environment that mimics in vivo conditions. Metastases are the primary cause of morbidity and mortality in cancer patients, and solid tumour metastases are mostly located in lymph nodes. Currently, there are no techniques that model the pre-metastatic lymph node microenvironment in vitro. In this study, we prepared a novel extracellular matrix, Lymphogel, which is derived from lymph nodes, mimicking the tumour microenvironment (TME) of metastatic carcinoma cells. We tested the suitability of the new matrix in various functional experiments and compared the results with those obtained using existing matrices.

Methods

We used both commercial and patient-derived primary and met

SUBMITTER: Naakka E 

PROVIDER: S-EPMC10424355 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature