Amyloid aggregates exert cell toxicity causing irreversible damages in the endoplasmic reticulum.

ABSTRACT: Amyloid oligomers and fibrils are protein aggregates that cause an onset and progression of many neurodegenerative diseases, diabetes type 2 and systemic amyloidosis. Although a growing body of evidence shows that oligomers and fibrils trigger mitochondrial dysfunction simultaneously enhancing production of reactive oxygen species, exact mechanisms by which these protein aggregates exert their toxicities remain unclear. In this study, we used advanced microscopic and spectroscopic methods to examine topography and structure of insulin aggregates grown in the lipid-free environment, as well as in the presence of major classes of phospho- and sphingolipids. We also employed a set of molecular markers to determine the extent to which insulin aggregates induce a damage of cell endoplasmic reti

SUBMITTER: Matveyenka M 

PROVIDER: S-EPMC10424722 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature