Intrinsic Disorder in the Human Tear Proteome.

ABSTRACT:

Purpose

We aimed to characterize the proteome of human tears and assess for the presence of intrinsically disordered proteins (IDPs). IDPs, despite lacking a rigid three-dimensional structure, maintain biological functionality and could shed light on the molecular interactions within tears.

Methods

We analyzed a dataset of 1475 proteins identified in the tear film of three healthy subjects. We employed several computational tools, including the Compositional Profiler, Rapid Intrinsic Disorder Analysis Online, Search Tool for the Retrieval of Interacting Genes, and Database of Disordered Protein Predictors to evaluate the intrinsic disorder, protein interactions, and functional characterization of the disordered regions within this proteome.

Results

Our analysis showed

SUBMITTER: Taylor Gonzalez DJ 

PROVIDER: S-EPMC10424804 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature