ScRNA-sequencing in chick suggests a probabilistic model for cell fate allocation at the neural plate border.

ABSTRACT: The vertebrate 'neural plate border' is a transient territory located at the edge of the neural plate containing precursors for all ectodermal derivatives: the neural plate, neural crest, placodes and epidermis. Elegant functional experiments in a range of vertebrate models have provided an in-depth understanding of gene regulatory interactions within the ectoderm. However, these experiments conducted at tissue level raise seemingly contradictory models for fate allocation of individual cells. Here, we carry out single cell RNA sequencing of chick ectoderm from primitive streak to neurulation stage, to explore cell state diversity and heterogeneity. We characterise the dynamics of gene modules, allowing us to model the order of molecular events which take place as ectodermal fates segregat

SUBMITTER: Thiery AP 

PROVIDER: S-EPMC10425176 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature