A Split CRISPR/Cas13b System for Conditional RNA Regulation and Editing.

ABSTRACT: The CRISPR/Cas13b system has been demonstrated as a robust tool for versatile RNA studies and relevant applications. New strategies enabling precise control of Cas13b/dCas13b activities and minimal interference with native RNA activities will further facilitate the understanding and regulation of RNA functions. Here, we engineered a split Cas13b system that can be conditionally activated and deactivated under the induction of abscisic acid (ABA), which achieved the downregulation of endogenous RNAs in dosage- and time-dependent manners. Furthermore, an ABA inducible split dCas13b system was generated to achieve temporally controlled deposition of m⁶A at specific sites on cellular RNAs through conditional assembly and disassembly of split dCas13b fusion proteins. We also showed t

SUBMITTER: Xu Y 

PROVIDER: S-EPMC10425183 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature