Programming conformational cooperativity to regulate allosteric protein-oligonucleotide signal transduction.

ABSTRACT: Conformational cooperativity is a universal molecular effect mechanism and plays a critical role in signaling pathways. However, it remains a challenge to develop artificial molecular networks regulated by conformational cooperativity, due to the difficulties in programming and controlling multiple structural interactions. Herein, we develop a cooperative strategy by programming multiple conformational signals, rather than chemical signals, to regulate protein-oligonucleotide signal transduction, taking advantage of the programmability of allosteric DNA constructs. We generate a cooperative regulation mechanism, by which increasing the loop lengths at two different structural modules induced the opposite effects manifesting as down- and up-regulation. We implement allosteric logic operatio

SUBMITTER: Liang Y 

PROVIDER: S-EPMC10425332 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature